Supporting information for Development of pipette tip
Electronic Supplementary Material (ESI) for Analytical Methods. This journal is © The Royal Society of Chemistry 2016 Supporting information for Development of pipette tip-based on molecularly imprinted polymer micro-solid phase extraction for selective enantioselective determination of (–)-(2S,4R) and (+)-(2R, 4S) ketoconazole in human urine samples prior to HPLC-DAD Ricky Cássio Santos da Silva1, Valdir Mano1, Arnaldo César Pereira1, Eduardo Costa de Figueiredo2, Keyller Bastos Borges*,1 1 Departamento de Ciências Naturais, Universidade Federal de São João del-Rei (UFSJ), Campus Dom Bosco, Praça Dom Helvécio 74, Fábricas, 36301-160, São João del-Rei, Minas Gerais, Brazil. 2 Faculdade de Ciências Farmacêuticas, Universidade Federal de Alfenas (UNIFAL- MG), Rua Gabriel Monteiro da Silva, 700, Centro, 37130-000, Alfenas, MG, Brazil. Figures Figure S1 Figure S1. Chemical structures of the cis-enantiomers of ketoconazole. Figure S2 Figure S2. TGA and DrTGA curves of (A) MIP and (B) NIP. Figure S3 Figure S3. Chromatogram referring to the enantioselective analysis of (+)-(2R,4S)-KTZ and (–)-(2S,4R)-KTZ of the urinary after single oral doses of 200 mg rac-KTZ to a healthy volunteer. Table Table S1. Chromatographic parameters to KTZ cis-enantiomers analysis under optimized conditions. Parameter (+)-(2R,4S)-KTZ (–)-(2S,4R)-KTZ Retention time (min) 5.38 6.91 %RSD (peak area) a 3.42 2.16 Af 1.44 1.38 Rs 3.69 K 2.69 3.81 α 1.42 N 3432 3550 a %RSD, relative standard deviation of all analyses expressed as a percentage; Af, asymmetric factor; Rs, resolution; k, retention factor (tm = 1.44 min, defined as the first significant baseline disturbance, corresponding to the retention time of a non-retained solute); α, separation factor; N, theoretical plates.