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Supporting information for Development of pipette tip

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Supporting information for Development of pipette tip
Electronic Supplementary Material (ESI) for Analytical Methods.
This journal is © The Royal Society of Chemistry 2016
Supporting information for
Development of pipette tip-based on molecularly imprinted polymer micro-solid
phase extraction for selective enantioselective determination of (–)-(2S,4R) and
(+)-(2R, 4S) ketoconazole in human urine samples prior to HPLC-DAD
Ricky Cássio Santos da Silva1, Valdir Mano1, Arnaldo César Pereira1, Eduardo Costa
de Figueiredo2, Keyller Bastos Borges*,1
1
Departamento de Ciências Naturais, Universidade Federal de São João del-Rei
(UFSJ), Campus Dom Bosco, Praça Dom Helvécio 74, Fábricas, 36301-160, São João
del-Rei, Minas Gerais, Brazil.
2
Faculdade de Ciências Farmacêuticas, Universidade Federal de Alfenas (UNIFAL-
MG), Rua Gabriel Monteiro da Silva, 700, Centro, 37130-000, Alfenas, MG, Brazil.
Figures
Figure S1
Figure S1. Chemical structures of the cis-enantiomers of ketoconazole.
Figure S2
Figure S2. TGA and DrTGA curves of (A) MIP and (B) NIP.
Figure S3
Figure S3. Chromatogram referring to the enantioselective analysis of (+)-(2R,4S)-KTZ
and (–)-(2S,4R)-KTZ of the urinary after single oral doses of 200 mg rac-KTZ to a
healthy volunteer.
Table
Table S1. Chromatographic parameters to KTZ cis-enantiomers analysis under optimized conditions.
Parameter
(+)-(2R,4S)-KTZ
(–)-(2S,4R)-KTZ
Retention time (min)
5.38
6.91
%RSD (peak area) a
3.42
2.16
Af
1.44
1.38
Rs
3.69
K
2.69
3.81
α
1.42
N
3432
3550
a %RSD, relative standard deviation of all analyses expressed as a percentage; Af, asymmetric factor;
Rs, resolution; k, retention factor (tm = 1.44 min, defined as the first significant baseline disturbance,
corresponding to the retention time of a non-retained solute); α, separation factor; N, theoretical plates.
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